Determination of the Total Mass of Antioxidant Substances and Antioxidant Capacity per Unit Mass in Serum Using Redox Titration

نویسندگان

  • Meijuan Zhang
  • Na Liu
  • Hui Liu
چکیده

Objective. Total antioxidant capacity in serum is determined by the total mass of antioxidant substances and the antioxidant capacity per unit mass (average activity). The purpose of this study was to develop a method to determine the mass of antioxidant substances and average activity in human serum. Methods. Specimens of serum were collected from 100 subjects each from two different age groups: over 75 years old and 20-40 years old. The test serum was diluted into a series of concentrations, following which standard oxidation agents (KMnO4 for potassium permanganate method and I2 for iodimetry) were added to each concentration of serum, and the absorbance of the mixture (optical density, OD) was measured. The OD value and logarithm of dilution factor (lgT) at the end of the titration were obtained, from which the lgT could be considered as mass of antioxidant substances (M). Total antioxidant capacity (Ta) was calculated with the equation Ta = 100/(OD1 + 2 ∗ OD2 + 2 ∗ OD3 + 2 ∗ OD4 + OD5), and average activity (A) was calculated as A = Ta/M. Results. The potassium permanganate method generated similar results to the iodimetric method. Compared with the younger group, total antioxidant capacity in the over-75-year age group was found to be significantly reduced, along with a decrease in the mass of antioxidant substances and average activity levels in human serum. Conclusions. The approach described in this paper is suitable for determining the average activity and mass of antioxidant substances in human serum.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Serum protein carbonyl and total antioxidant capacity levels in pemphigus vulgaris and bullous pemphigoid

Background: Pemphigus diseases including pemphigus vulgaris (PV) and bullous pemphigoid (BP) are autoimmune diseases that cause severe blistering of the skin and mucous membranes. Among inflammatory mediators, reactive oxygen species (ROS) are involved in the pathogenesis of a wide variety of diseases through oxidative stress for which protein carbonyl (PC) and total antioxidant capacity (TAC) ...

متن کامل

The Correlation Between Serum Vitamin D Level and Total Antioxidant Capacity in diabetic and Non-diabetic Subjects in Iran

Background& Objective:  Increase in intra- and extracellular glucose levels can cause oxidative stress, and the prolonged imbalance between prooxidants and antioxidantscan lead to cell damage and the associated complications in patients with diabetes. Vitamin D acts as a strong antioxidant in the body and several studies emphasized on its important rol...

متن کامل

Investigating the protective effect of Satureja khuzestanica Jamzad essential oil and carvacrol on lipid peroxidation, antioxidant enzymes, glutathione, and total antioxidant capacity in the plasma of diabetic rats

Background: Type 2 diabetes is one of the main causes of death and its treatment is one of the clinical priorities. This study aimed to evaluate the effect of Satureja khuzistanica essential oil (SKEO) and carvacrol on lipid peroxidation index (MDA), plasma total antioxidant capacity (TAC), and also the activity of antioxidant enzymes. Materials and Methods: The study was experimental in natur...

متن کامل

Simple and Sensitive Method of Fluorometry for Determination of Total Antioxidant Capacity

Background and Objective: Oxidative stress plays an important role in the pathogensis of various diseases, including lung cancer, chronic obstructive pulmonary disease, and atherosclerosis. Total antioxidant capacity plays a significant role in the body’s antioxidant defense, so its assessment is a matter of the utmost importance. Currently, assessment of this capacity is performed in var...

متن کامل

Evaluation of TRAP and FRAP Methods for Assessing Total Antioxidant Capacity of Rat Serum

Tayefi-Nasrabadi H1, Khodarahmi R2 1. Assistant Professor, Department of Biochemistry, Faculty of Veterinary Medicine, Tabriz University 2. Assistant Professor, Department of Biochemistry, Faculty of Medicine, Kermanshah University of Medical Sciences Abstract Background: Because of the difficulty in measuring each antioxidant component separately and the interactions among different...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2014  شماره 

صفحات  -

تاریخ انتشار 2014